THE REVERSIBLE INACTIVATION OF TOBACCO MOSAIC VIRUS BY CRYSTALLINE RIBONUCLEASE

The Reversible Inactivation of Tobacco Mosaic Virus by Crystalline Ribonuclease

The reversible inactivation of tobacco mosaic virus by crystalline ribonuclease is reported. Studies on the effect of time of standing on the amount of inactivation, and on the effect of dilution and repeated high speed centrifugation on the recovery of virus activity, and the preparation of an insoluble virus-enzyme complex show that the inactivation is brought about at least in part by a comb...

The isolation and properties of crystalline tobacco mosaic virus

The Ultraviolet Light and Photosensitized Inactivation of Tobacco Mosaic Virus

The quantum yield for the inactivation of tobacco mosaic virus has been determined at 253.7 mmicro and found to be 4.3 x 10(-6). The possible significance of the observed one-hit process of inactivation has been discussed in terms of the kinetics and the rupture of model substances including nucleic acid. The ultraviolet light inactivation, which proceeds independent of oxygen, occurs without c...

Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The...

Quantum yields for inactivation of tobacco mosaic virus nucleic acid by ultraviolet radiation (254 nm.).

The quantum yield, under non-photoreactivating conditions, for inactivation of tobacco mosaic virus RNA by ultraviolet radiation (254 nm.) is independent of the RNA concentration but varies appreciably with the ionic strength of the irradiation solvent, being approximately three times greater in water than in o-I M-potassium phosphate buffer. Photoreactivation of inactivated tobacco mosaic viru...